Comic Book: Marvel Comics: "For a Few Fists More! (March, 1975)
Witnessing Iron Fist fight off a bunch of crooks, Peter Parker decides to change into Spider-Man to get some more photos of Iron Fist. The two heroes soon become the target of Drom, the Backwards Man, who hopes to use the two of them to help halt his reverse ageing process.
After capturing Spider-Man he explains his story -- how after his birth he suddenly was replaced by an old man, and has since been ageing backwards. Iron Fist tracks down Spider-Man and Drom before Drom can use his metabolic disruptor device on the web-slinger. Fighting Drom, they break his mirror over him, which causes him to rapidly reverse age until he no longer exists. After the battle, Iron Fist wonders if because of the nature of Drom, they will remember their encounter. This proves to be prescient, as later Peter Parker cannot remember what happened.
http://marvel.wikia.com/wiki/Drom_(Earth-616)
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Amazing Spider-Man #75
Comic Book: Marvel Comics: [Magazine Management Co.] "Death Without Warning!" (08/30/1969)
Gang boss Silvermane tests an ancient Egyptian formula. After fighting Spidey, his curse is revealed (AR past birth).
Having drank the elixir of life, Silvermane has reversed his age to that of a man in his prime. When Ceasar tries to convince Marko that the young man is not Silvermane but an impostor, Silvermane lashes out at them. Spider-Man has to fight Silvermane, who has de-aged once more to a man in his twenties. Connors cannot hold back the transformation any longer, and changes back into the Lizard monster he loathes. As Spider-Man fights him, Silvermane is shocked to find that he's still getting younger and flees the room. Spider-Man finishes off the rest of the Maggia grunts, and watches as the once fearsome crime boss de-ages past infancy until he finally becomes nothing but a memory.
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Spider-Man and Power Pack #1
Marvel Comic Book: "The New Kid" Part 1: Everything Old is Young Again (11/06/2006)
Spider-Man is exposed to "Age-Reversing goop" and becomes a pre-teen version of himself. (Male Ar)
-- Visible Time & SKJAM
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Spider-Man and Power Pack #2
Marvel Comic Book: "The New Kid" Part 2: Sands Of Time (12/06/2006)
In order to get around, Peter must don a kiddie costume. When he reverses the AR, that costume is a little...tight. (male AP RN UC, male AR)
-- SKJAM
SPIDER-MAN
Cartoon series: Season 01 Episode #18?: "Fountain of Terror" (1967-1968)
Dr. Curtis Conner goes missing in the Florida swamps after finding the Fountain of Youth. When Spidey investigates the scientist's disappearance, he discovers a fifteenth century Spanish conquistador, Ponce de Leon, who is intent upon keeping the magical fountain a secret. Harley Clivendon is in the area too, determined to find Conner and the perpetual-youth-endowing waters.
Spidey and Conner's son, Billy, escape quicksand, alligators, and Clivendon's arrows, to arrive at a Spanish fort, where Conner is being held captive by de Leon. At the fort, Billy frees his father while Spidey duels with Clivendon and de Leon prepares to fire a cannon at his unwelcome guests.
Spidey webs Clivendon, and Billy, seeing de Leon igniting the cannon, saves Spidey's life by directing the firing cannon upward. The cannonball drops on the nearby fountain and destroys it, and de Leon flees into the forest. (AS)
-- Jeff
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Spider-Man (2003)
Cartoon Series: Season 01; Episode #02: "Sword of Shikata" (07/12/03)
Spider-Man encounters a woman hired to capture him for a rare animal collector. She looks young but a sword she uses gives her youth, without it she is really an old woman. At the end spider-Man destroys the sword and she turns back into an old woman and dies.
-- Arfreak
Spider-Man: The Animated Series
Cartoon Series: Season 02; Episode #10: "Neogenic Nightmare Part 12: Ravages Of Time" ("Sins of the Father Chapter XIII: The Tablet of Time")
It's a double double-cross. Doc Connors warns his captor Silvermane about the tablet, but Silvermane won't listen and positions the ancient stone in a replica of the Toltec temple. As the sun rises and courses through the stone, the beam engulfs Silvermane. Suddenly, he's a young man again. Connors' fury triggers his own metamorphosis into The Lizard and in the ensuing battle with Tombstone and Spider-Man, Silvermane morphs again, younger and younger until he is a infant.
-- TBTC
Spider-Man: The Animated Series
Cartoon Series: Season 02; Episode #13: "Neogenic Nightmare Part 13: Shriek Of The Vulture"
While CEO Adrian Toomes is absorbed in regaining his youth, Norman Osborn is engineering a hostile takeover of Toomes Aeronautical. Toomes vows revenge and takes to the air as the Vulture, armed with youth-absorbing talons that temporarily make him young. Meanwhile, on campus, Peter arrives to see Mary Jane in Faust where Harry Osborn is snatched out of the audience by the Vulture. Spidey rescues Harry, but the Vulture escapes. Later, as Toomes' talons grip Spider-Man's shoulder, Spidey's strength and vitality fade while the Vulture revels in newfound youth and super strength. To make his victory complete, Toomes begins to rip off Spidey's mask ....
-- TBTC
Spider-Man: The Animated Series
Cartoon Series: Season 02; Episode #14: "Neogenic Nightmare Part 14: The Final Nightmare"(Partners in danger IV)
The shocking conclusion to the "Neogenic Nightmare" story-line!! Spider-Man seeks Curt Connors aid to help reverse the aging effects of the Vulture's talons. The Vulture meanwhile sporadically turns into the man-spider from the mutagenics he absorbed with Spidey's youth.
At the same time, the Scorpion grabs Dr. Farley Stillwell to seek a cure for his own mutation. With Doc Connors in the middle, feeling the onset of his own his reptilian transformation, will everyone be cured in time for Peter to meet up with Mary Jane as she confronts her wayward father?
-- TBTC
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Ultimate Spider-Man
Cartoon TV Series: Season 01; Episode #17: "Snow Day" (08/05/2012)
Agent Coulson orders Spider-Man and his team to report to a S.H.I.E.L.D. Arctic base for winter survival training. They instead go against their orders and sneak off to a deserted tropical island (which Nova finds on the S.H.I.E.L.D. database) for a vacation. However, while the island seems like a beautiful paradise, something sinister is lurking in the sand, and does not want his new "play-mates" to leave again.
Spider-Man, Nova, Luke Cage, Iron Fist and White Tiger go to a deserted island listed in the SHIELD data_base_ instead of going on snow training exercises. There they encounter a young boy who says he is with his family. They then encounter his older brother who looks a lot like him named Flint who both can control the sand. It turns out they are on person in the villain "The Sandman" who uses the form of the boy so he wouldn’t be lonely
-- Tazz
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Ultimate Spider-Man
Cartoon TV Series: Season 02; Episode #03: "The Rhino" (01/27/2013)
Spider-Man and Power Man come into conflict with the Rhino, an immensely strong rhinoceros-themed villain, who tries to steal from Oscorp's chemical shipments. At school, Peter and Luke notice Flash Thompson is bullying a shy, nerdy classmate named Alex, but they soon discover Alex's shocking connection to the Rhino and the lengths he will go to in order to get revenge on Flash.
Spider-Man and Luke Cage battle Rhino (if you don't know who he is a huge muscular man in a rhino costume) who has been stealing formulas for Oscorp which he calls his "medicine". It turns out Rhino is Alex a skinny teenage nerd who turns into the Rhino so he can get revenge on the guy who bullies him, Flash Thompson.
Well anyway there was also a minor sort of ar. While seeing Flash try to flee from Rhino. Spidey comments that he is screaming like a baby. He then imagines Flash as a baby and him taking care of him.
-- Tazz
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Ultimate Spider-Man
Cartoon TV Series: Season 02; Episode #12: "Itsy Bitsy Spider-Man" (06/09/2013)
While looking for Thor missing in action, Spider-Man's team is shrunk into super-deformed versions of their own selves by one of Loki's tricks. Nick Fury has Spider-Man's team placed in the Helicarrier's D.A.Y.C.A.R.E. area, but with Thor also having been shrunk, Spider-Man's team must find a way to help Thor fight Loki and the Destroyer Armor and return to normal size.
-- Tazz